Adenovirus Preparations

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Adenovirus Preparations
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The University of Pittsburgh Vector Core carries a variety of stock adenoviruses for distribution to registered researchers, and offers a variety of other services involving adenoviruses:

Services & Pricing
 stock
1 vial of 1x10e11 viral particles of this vector42
5 vials of 1x10e11 viral particles of this vector185
10 vials of 1x10e11 viral particles of this vector320
25 vials of 1x10e11 viral particles of this vector575
Medium production: 10x15cm plate production, typically yielding 1x10e13 viral particles925
Large production: One cell factory, yielding from 1 to 4x10e13 viral particles1196
 expansions
Medium scale expansion of your adenovirus or lysate. This is a 10x15cm plate production, typically yielding 1x10e13 viral particles.925
Large scale expansion of your adenovirus or lysate. This is one cell factory, typically yielding from 1 to 4x10e13 viral particles.1196
 cloning services & productions
Adenoviral cloning, when you supply your gene of interest ready for direct insertion into our shuttle plasmid626
Adenoviral cloning, when you supply your gene of interest not ready for direct insertion into our shuttle plasmid876
Adenoviral cloning, when you do not have the gene of interest, and for siRNA sequence cloningCall for price
Medium scale production of recombinant Adenovirus from shuttle plasmid. This is a 10x15cm plate production, typically yielding 1x10e13 viral particles. We cannot guarantee the amounts produced or that the resulting product will be non-toxic. We will make two attempts, and if you wish that we continue after that, the work will be charged regardless of the outcome.1860
Large scale production of recombinant Adenovirus from shuttle plasmid. This is one cell factory, typically yielding from 1 to 4x10e13 viral particles. We cannot guarantee the amounts produced or that the resulting product will be non-toxic. We will make two attempts, and if you wish that we continue after that, the work will be charged regardless of the outcome.2130
 optional add-on quality control
RCA testing of this adenoviral vector, available for medium or large expansions or productions.190
Sequencing of this adenoviral vector, available for medium or large expansions or productions.80
Sterility testing of this adenoviral vector, available for medium or large expansions or productions.100

 

Note on optional services for medium or large productions or expansions of stock or novel viruses:

RCA test
  Detection of Replication Competent Adenovirus:

The Vector Core will test 1x10E11 viral particles for the presence of replication competent adenovirus. The possible conclusions include no RCA, minimal/low RCA, or high RCA. The Vector Core can help the researcher interpret the results of the RCA testing and offer suggestions for the proper usage of positive samples.
 
Sequencing test
  The Vector Core will sequence the first 400 base pair and the last 400 base pair of your insert containing a CMV promoter to identify the product. The Vector Core can make arrangements for full length sequencing if the investigator requires it. The price for full length sequencing will be assessed on an individual basis at the time of the request.  
Sterility test
  The Vector Core will test 1x10e11 viral particle for the presence of microbial contamination.  

 

Credits: WWW implementation by Andrea Gambotto of University of Pittsburgh / Department of Surgery and Molecular Genetics and Biochemistry / Pittsburgh Human Gene Therapy Center / Pittsburgh and The Bajzek Group, Inc.

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